Biomedical Science and Engineering Project 1: Prostate Cancer
BSE Research Project #1: Metabolic and epigenetic insights of PFAS and high fat diet-induced prostate carcinogenesis
Key Aims: (1) Delineate the impact of PFAS and high fat diet on prostate tumorigenicity and cancer aggressiveness, (2) Define the metabolic signature of PFAS and high fat diet-treated benign and cancerous prostate cells, and (3) Determine how PFAS exposure and high fat diet impact the epigenetic landscape and PPARa signaling. BSE1 will study PFAS- and diet-induced changes in PPARα signaling and glycolytic pathways; metabolomic and epigenetic indicators, and histone (H3K4, H3K9 and H3K27) methylation and acetylation.
Disease Mechanisms and Model Systems: Model organisms include in vitro cultures and in vivo xenografts using disease-free human prostate epithelial cells and human prostate cancer models. We will study how high-fat diets exacerbate the carcinogenic effects of PFAS in human prostate spheroids/organoids and xenografts.
Study Sites/Community: UIUC, University of Chicago, UC Riverside, CCIL, Carle Foundation Hospital (Urbana).
Integration: Environmental Science and Engineering Project 1 informs BSE RPs on priority compounds to test and Environmental Science and Engineering Project 2 provides BSE1 products from the treatment technologies for cancer evaluation. Based on toxicity/adverse effects Environmental Science and Engineering Project 2 will optimize treatment conditions. The Research Experience and Training Coordination Core will work with Administrative Core on translation and Community Engagement Core on cancer risk reduction health communication messages. Data Management and Analysis Core and Analytical Services Core will be used for experiments, reagent sharing, data management and analysis.