Analytical Services Core
Overarching goal: To train CEPaCT researchers in state-of-the-art instrumentation, develop novel tools, enhance collaborations, and coordinate sharing and standardization of compounds, protocols, and samples.
Approach: (1) Utilize advanced instrumentation and analytical techniques for quantitative and discovery-based studies, (2) Develop innovative tools for high resolution screening and quantification of biomarkers, and (3) Coordinate core interactions and data handling procedures with the center. While Data Management and Analysis Core is the hub for data management and analysis, ASC will serve as a hub for sample preparation, experimental design, and protocol standardization. ASC will work closely with the institutional facilities (directors of these facilities are Co-Is of ASC) to enable the research projects and cores. Data Management and Analysis Core and ASC share a Project Manager.
Select Facilities and Resources for ASC
Tumor Engineering and Phenotyping Core (TEP)
DNA Services – Roy J. Carver Biotechnology Center
Metabolomics Services - Roy J. Carver Biotechnology Center
Clarkson University (Prof. Yang – Project 2)
Duke University (Prof. Ferguson – Project 1 and ASC)
Select PFAS publications:
Sands, M., Zhang, X., Spinella, M., Madak-Erdogan, Z., and Irudayaraj, J. Comparative Hepatotoxicity of Novel Lithium Bis(trifluoromethanesulfonyl)imide (LiTFSI) and Legacy Perfluorooctanoic Acid (PFOA) in Male Mice: Insights into Epigenetic Mechanisms and Pathway-Specific Responses. 2024. Environment International, 108556. https://doi.org/10.1016/j.envint.2024.108556
Zhang, X., Sands, M., Lin, M., Guelfo, J., and Irudayaraj, J. In vitro toxicity of Lithium Bis(trifluoromethanesulfonyl)imide (LiTFSI) on human renal and hepatoma cells. 2024. Toxicology Reports. 12: 280-288. doi: 10.1016/j.toxrep.2024.02.008
Boyd, R., Shokry, D., Fazal, Z., Rennels, B., Freemantle, S., La Frano, M., Prins, G., Madak Erdogan, Z., Irudayaraj, J., Singh, R., Spinella, M. Perfluorooctanesulfonic acid alters pro-cancer phenotypes and metabolic and transcriptional signatures in testicular germ cell tumors. 2024. Toxics, 12(4), 232. https://doi.org/10.3390/toxics12040232
Sands, M., Zhang, Z., Jensen, T., La Frano, M., Lin, M., Koester, B., and Irudayaraj, J. PFAS assessment in fish: samples from Illinois waters. 2024. Science of the Total Environment, 172357. doi: 10.1016/j.scitotenv.2024.172357
Sands, M., Zhang, X., and Irudayaraj, J. Kidney toxicology of a novel compound HQ-115 used in energy applications: an epigenetic evaluation. ACS Environmental Science and Technology (In Review) 2024 (bioRXiv doi: https://doi.org/10.1101/2024.04.02.587863)
Zhang, X., Sands, M., La Frano, M., Spinella, M., Masoud, F., Fields, C., Madak-Erdogan, Z., Jensen, T., and Irudayaraj, J. MicroRNA’s and PFAS: A pilot study in blood collected from firefighters. 2024. bioRXiv: doi: https://doi.org/10.1101/2024.04.05.588341.
Zhang X, Flaws JA, Spinella MJ, Irudayaraj J. The Relationship between Typical Environmental Endocrine Disruptors and Kidney Disease. Toxics. 2023;11(1):32. PubMed PMID: doi:10.3390/toxics11010032.
Shah MM, Ahmad K, Boota S, Jensen T, La Frano MR, Irudayaraj J. Sensor technologies for the detection and monitoring of endocrine-disrupting chemicals. Frontiers in Bioengineering and Biotechnology. 2023;11. doi: 10.3389/fbioe.2023.1141523.
Rashid F, Dubinkina V, Ahmad S, Maslov S, Irudayaraj JMK. Gut Microbiome-Host Metabolome Homeostasis upon Exposure to PFOS and GenX in Male Mice. Toxics. 2023;11(3):281. PubMed PMID: doi:10.3390/toxics11030281.
Liu W, Zhang X, Wen Y, Anastasio MA, Irudayaraj J. A machine learning approach to elucidating PFOS-induced alterations of repressive epigenetic marks in kidney cancer cells with single-cell imaging. Environmental Advances. 2023;11:100344. doi: https://doi.org/10.1016/j.envadv.2023.100344.
Jeong Y, Vyas K, Irudayaraj J. Toxicity of per- and polyfluoroalkyl substances to microorganisms in confined hydrogel structures. Journal of Hazardous Materials. 2023;456:131672. doi: https://doi.org/10.1016/j.jhazmat.2023.131672.
2022
Wen Y, Rashid F, Fazal Z, Singh R, Spinella MJ, Irudayaraj J. Nephrotoxicity of perfluorooctane sulfonate (PFOS)-effect on transcription and epigenetic factors. Environ Epigenet. 2022;8(1):dvac010. Epub 2022/06/01. doi: 10.1093/eep/dvac010. PubMed PMID: 35633893; PMCID: PMC9134076.
Rashid F, Dubinkina V, Maslov S, Irudayaraj J, editors. North American Travel Grant Recipient: Effect of Subacute Exposure to PFAS Compounds on Gut Microbial Functions and Liver Metabolome in Mice. INTERNATIONAL JOURNAL OF TOXICOLOGY; 2022: SAGE PUBLICATIONS INC 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA.
Boyd RI, Ahmad S, Singh R, Fazal Z, Prins GS, Madak Erdogan Z, Irudayaraj J, Spinella MJ. Toward a Mechanistic Understanding of Poly- and Perfluoroalkylated Substances and Cancer. Cancers (Basel). 2022;14(12). Epub 2022/06/25. doi: 10.3390/cancers14122919. PubMed PMID: 35740585; PMCID: PMC9220899.
2021
Rashid F, Irudayaraj J, editors. North American Travel Grant Effect of PFOA on DNA Methylation and Tight Junctions in Mouse Colon Tissues. INTERNATIONAL JOURNAL OF TOXICOLOGY; 2021: SAGE PUBLICATIONS INC 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA.
Imir OB, Kaminsky AZ, Zuo QY, Liu YJ, Singh R, Spinella MJ, Irudayaraj J, Hu WY, Prins GS, Madak Erdogan Z. Per- and Polyfluoroalkyl Substance Exposure Combined with High-Fat Diet Supports Prostate Cancer Progression. Nutrients. 2021;13(11). Epub 2021/11/28. doi: 10.3390/nu13113902. PubMed PMID: 34836157; PMCID: PMC8623692.
Ahmad S, Wen Y, Irudayaraj JMK. PFOA induces alteration in DNA methylation regulators and SARS-CoV-2 targets Ace2 and Tmprss2 in mouse lung tissues. Toxicology Reports. 2021;8:1892-8. doi: https://doi.org/10.1016/j.toxrep.2021.11.014.
2020
Wen Y, Mirji N, Irudayaraj J. Epigenetic toxicity of PFOA and GenX in HepG2 cells and their role in lipid metabolism. Toxicology in Vitro. 2020;65:104797. doi: https://doi.org/10.1016/j.tiv.2020.104797.
Wen Y, Chen J, Li J, Arif W, Kalsotra A, Irudayaraj J. Effect of PFOA on DNA Methylation and Alternative Splicing in Mouse Liver. Toxicology Letters. 2020;329:38-46. doi: https://doi.org/10.1016/j.toxlet.2020.04.012.
Rashid F, Ahmad S, Irudayaraj JMK. Effect of Perfluorooctanoic Acid on the Epigenetic and Tight Junction Genes of the Mouse Intestine. Toxics. 2020;8(3):64. PubMed PMID: doi:10.3390/toxics8030064.
Rashid F, Ramakrishnan A, Fields C, Irudayaraj J. Acute PFOA exposure promotes epigenomic alterations in mouse kidney tissues. Toxicology Reports. 2020;7:125-32. doi: https://doi.org/10.1016/j.toxrep.2019.12.010.
Liu W, Irudayaraj J. Perfluorooctanoic acid (PFOA) exposure inhibits DNA methyltransferase activities and alters constitutive heterochromatin organization. Food and Chemical Toxicology. 2020;141:111358. doi: https://doi.org/10.1016/j.fct.2020.111358.
Jabeen M, Fayyaz M, Irudayaraj J. Epigenetic Modifications, and Alterations in Cell Cycle and Apoptosis Pathway in A549 Lung Carcinoma Cell Line upon Exposure to Perfluoroalkyl Substances. Toxics. 2020;8(4):112. PubMed PMID: doi:10.3390/toxics8040112.